The Fluorescent Measurement in D. Magna Feeding Suppression Bioassay during the Pre-exposure in Potassium Dichromate for 1, 2, 3, 4 and 5 Hours
Kamaya Minori1, Ginatullina Elena2, Yamagata Kohei3

1Kamaya Minori * Department of Applied Chemistry, Kogakuin University, Tokyo, Japan.
2Ginatullina Elena* Department of Ichtyology and Hydrobiology, Institute of Zoology Uz Academy of Sciences, Tashkent, Uzbekistan.
3Yamagata Kohei Department of Applied Chemistry, Kogakuin University, Tokyo, Japan.
Manuscript received on February 01, 2020. | Revised Manuscript Received on February 07, 2020. | Manuscript published on February 20, 2020. | PP: 1-4 | Volume-2 Issue-10, February 2020. | Retrieval Number: I0141122919/2020©LSP | DOI: 10.35940/ijbsac.I0141.0221020
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Abstract: The present study focused on observation of effect of potassium dichromate as standard toxicant on feeding activity of D. magna. Isolated feeding suppression was measured as fluorescence intensity of ingested microbeads by daphnids and expressed as its feeding rate (EC50FI). We calculated the sub-lethal feeding suppression endpoints for 1, 2, 3, 4 and 5 hours exposure in potassium dichromate and compared these values with EC50 concentration of immobilization test of D. magna. The most close to immobilization (EC50)result was obtained as for 4 as well for 5 hours exposure time; however 4 hs exposure was chosen as more adequate exposure time for our design of feeding suppression bioassay.
Keywords: Acute test, feeding rate, fluorescent intensity, polyester microbeads.